Tag

Part:BBa_K3385056:Design

Designed by: Lucas Levassor   Group: iGEM20_DTU-Denmark   (2020-10-11)


SigPuppy


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The long unpaired primers used were considered to be a problem when performing PCR for amplification. Other methods like MoClo also have to be considered to ensure efficient modulation for future use.

Source

This is a synthetic signal peptide which was obtained by DNA synthesis.

References

[1] Jose Juan Almagro Armenteros, Casper Kaae Sønderby, Søren Kaae Sønderby, Henrik Nielsen, Ole Winther; DeepLoc: prediction of protein subcellular localization using deep learning, Bioinformatics, btx431